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l2 rat lung cells atcc ccl 149  (ATCC)


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    ATCC l2 rat lung cells atcc ccl 149
    L2 Rat Lung Cells Atcc Ccl 149, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l2+rat+lung+cells+atcc+ccl+149/L2/pm40143360-54-40-44
    Average 96 stars, based on 459 article reviews
    l2 rat lung cells atcc ccl 149 - by Bioz Stars, 2026-09
    96/100 stars

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    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Novel Rodent Coronavirus-like Virus Detected Among Beef Cattle with Respiratory Disease in Mexico
    Article Snippet: The cells used for virus isolation were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. To isolate the rodent coronavirus from the pan-CoV RT-PCR positive nasal and bioaerosol specimens, we inoculated monolayers of Vero-E6 (African green monkey kidney cells; ATCC; CRL-1586), LLC-MK2 (Rhesus monkey kidney cells; ATCC; CCl-7), MDBK (Madin–Darby bovine kidney; ATCC; CCL-22), and L2 (rat lung cells; ATCC; CCL-149). .. Briefly, Vero-E6, LLC-MK2, and MDBK cells were propagated in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM, ThermoFisher Scientific, Waltham, MA, USA, cat no. 11965092), whereas the L2 cells were cultured in Ham’s F-12K (Kaighn’s) medium (F-12K; ThermoFisher Scientific, cat no. 21127022).

    Article Title: Novel Rodent Coronavirus-like Virus Detected Among Beef Cattle with Respiratory Disease in Mexico.
    Article Snippet: The cells used for virus isolation were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. To isolate the rodent coronavirus from the pan-CoV RT-PCR positive nasal and bioaerosol specimens, we inoculated monolayers of Vero-E6 (African green monkey kidney cells; ATCC; CRL-1586), LLC-MK2 (Rhesus monkey kidney cells; ATCC; CCl-7), MDBK (Madin–Darby bovine kidney; ATCC; CCL-22), and L2 (rat lung cells; ATCC; CCL-149). .. Briefly, Vero-E6, LLC-MK2, and MDBK cells were propagated in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM, ThermoFisher Scientific, Waltham, MA, USA, cat no. 11965092), whereas the L2 cells were cultured in Ham’s F-12K (Kaighn’s) medium (F-12K; ThermoFisher Scientific, cat no. 21127022).



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    FIG. 3. Functional analysis of the promoter elements of the rat type III hexokinase gene. The various deletion constructs included sequence shown at the left, inserted into the luciferase reporter vector, pGL2-Basic. The constructs pD4 and pINRD contained site-directed mutations at Oct-1 and Inr sites, respectively (see text). Firefly lucifer- ase activities expressed <t>in</t> <t>PC12</t> (Fig. 3A) or <t>L2</t> (Fig. 3B) cells trans- fected with the reporter constructs have been normalized on the basis of Renilla luciferase activity encoded by the co-transfected control plas- mid, pRL-CMV. Results are the mean 6 S.D. from at least six samples from two independent transfection experiments.
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    FIG. 3. Functional analysis of the promoter elements of the rat type III hexokinase gene. The various deletion constructs included sequence shown at the left, inserted into the luciferase reporter vector, pGL2-Basic. The constructs pD4 and pINRD contained site-directed mutations at Oct-1 and Inr sites, respectively (see text). Firefly lucifer- ase activities expressed <t>in</t> <t>PC12</t> (Fig. 3A) or <t>L2</t> (Fig. 3B) cells trans- fected with the reporter constructs have been normalized on the basis of Renilla luciferase activity encoded by the co-transfected control plas- mid, pRL-CMV. Results are the mean 6 S.D. from at least six samples from two independent transfection experiments.
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    FIG. 3. Functional analysis of the promoter elements of the rat type III hexokinase gene. The various deletion constructs included sequence shown at the left, inserted into the luciferase reporter vector, pGL2-Basic. The constructs pD4 and pINRD contained site-directed mutations at Oct-1 and Inr sites, respectively (see text). Firefly lucifer- ase activities expressed in PC12 (Fig. 3A) or L2 (Fig. 3B) cells trans- fected with the reporter constructs have been normalized on the basis of Renilla luciferase activity encoded by the co-transfected control plas- mid, pRL-CMV. Results are the mean 6 S.D. from at least six samples from two independent transfection experiments.

    Journal: The Journal of biological chemistry

    Article Title: Characterization of the rat type III hexokinase gene promoter. A functional octamer 1 motif is critical for basal promoter activity.

    doi: 10.1074/jbc.274.44.31700

    Figure Lengend Snippet: FIG. 3. Functional analysis of the promoter elements of the rat type III hexokinase gene. The various deletion constructs included sequence shown at the left, inserted into the luciferase reporter vector, pGL2-Basic. The constructs pD4 and pINRD contained site-directed mutations at Oct-1 and Inr sites, respectively (see text). Firefly lucifer- ase activities expressed in PC12 (Fig. 3A) or L2 (Fig. 3B) cells trans- fected with the reporter constructs have been normalized on the basis of Renilla luciferase activity encoded by the co-transfected control plas- mid, pRL-CMV. Results are the mean 6 S.D. from at least six samples from two independent transfection experiments.

    Article Snippet: Materials—PC12 (rat adrenal pheochromocytoma) and L2 (rat lung) cell lines were obtained from American Type Culture Collection (Manassas, VA).

    Techniques: Functional Assay, Construct, Sequencing, Luciferase, Plasmid Preparation, Activity Assay, Transfection, Control